Effect of Unpalatable Diets, Food Restriction and Saccharin-Adulterated Diet on Tryptic, Chymotryptic, and Amylolytic Activity in Pancreas, Intestine and Feces of Rats1′2 MICHAEL NAIM … … JOSEPHG. BRANDtt ANDMORLEYR. KAREt’Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel and îMonell Chemical Senses Center and\Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104 ABSTRACT Growing rats were fed either 10 or 17%protein (casein) diets whose

ABSTRACT Growing rats were fed either 10 or 17%protein (casein) diets whose taste was changed daily by addition of aversive stimuli. Under these circumstances food intake and body weight gain were reduced compared with controls fed unadulterated diets. More amylolytic activity per gram pancreas was found in rats feda 10%protein, aversive diet and in their pair-fed controls fed restricted amounts of an unadulterated diet compared with ad libitum controls. More amylolytic activity per gram chyme of small intestine was also found in rats fed less than ad libitum amounts ofa 10%protein diet. The chymotrypsin activity in the pancreas of rats feda 17%protein, aversive diet was greater than in that of control animals or that of the pair-fed animals. The 2-to 10-fold higher proteolytic activity found in the small intestine and especially in the large intestine and feces of rats fed the aversive diet wasspecificially related to the adulteration of the diet with sodium saccharin. Feeding a diet containing sodium sac charinledtolower cecalpH. Under conditions of the present experiments, changes in digestive enzyme activity are not directly related to the aversive taste of the diet. J. Nutr. 112: 2104-2115,1982. INDEXING KEYWORDS aversive taste •unpalatable diets•food restriction •saccharin•trypsin•chymotrypsin•amylase ….. portantrolefor oral stimulation inpatients who are tube-fed into the stomach orintes tine (6,7). However, nitrogen balance ex periments performed on human subjects (8) and rats (9) fed unpalatable diets indicated no change indigestibility, and the lower feed efficiency values observed in rats fed an un palatable diet (10) were found to be second aryeffectsdueto the reduction in food intake (9). The present studies were carried out to directly determine whether, under adlibi tumfeeding unpalatable diets can affect digestive enzyme content in the pancreas and along the intestinal tract of rats. The results demonstrate that amylolytic activity isaf fectedbyfood restriction, whether or not that restriction is imposed directly, as in offering an amount of food below the ad libitum level, or is achieved by offering ad libitum a diet that the animals find aversive. In addition, increases in proteolytic enzyme activities in the large intestine are found for the aversive diet, and this increase is specifically related to the presence of saccharin in the diet.

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